Dr.Thanayod Sasivimolrattana
Lect. (Department of Microbiology, Faculty of Public Health, Mahidol University)
About me
BIOGRAPHY
In a world teeming with unseen wonders and complex organisms, Thanayod Sasivimolrattana, Ph.D. emerged as a passionate explorer of the microbial realm. My journey, marked by an insatiable curiosity and unyielding dedication, has led me to uncover the secrets of microbes that hold profound implications for human health. The foundations of my journey were laid at the prestigious King Mongkut’s Institute of Technology Ladkrabang, where I earned a B.Sc. in Industrial Microbiology. During this phase, my fascination with the potential of microorganisms to create impactful substances was ignited. My research at this stage focused on the screening of Streptomyces spp. strains with the capability to produce antimicrobial compounds. With a hunger for deeper insights, I pursued a M.Sc. in Medical Microbiology at Chulalongkorn University, Thailand. Here, my focus shifted to the realm of medical virology. The research took me into the intricate mechanisms of herpes simplex virus (HSV) infection in activated T lymphocytes. Undeterred by the challenges of a rigorous academic path, I earned a Ph.D. in Medical Microbiology (International Program) from the same esteemed institution. My research evolved into a comprehensive exploration of the human papillomavirus (HPV) and its intricate association with cervical cancer. There were two parts of my doctoral work: the research unraveled the multifaceted role of the microbiome (Bacteriome, Mycobiome, and Virome) in HPV-related cervical cancer while dissecting the functions of the pivotal HPV16 E1 protein in cervical carcinogenesis. These groundbreaking insights contributed significantly to our understanding of the factors underpinning this complex and devastating disease. My journey transcends academia, extending into a dynamic and impactful professional realm. Currently a lecturer in the Department of Microbiology at the Faculty of Public Health, Mahidol University, my expertise spans an array of many research areas, including anti-viral drug screening, cell-based evaluation of novel anticancer drug screening, molecular mechanisms of viral infection, and cancer development, as well as the exploration of bacteriome, mycobiome, and virome in diverse settings.
In a world teeming with unseen wonders and complex organisms, Thanayod Sasivimolrattana, Ph.D. emerged as a passionate explorer of the microbial realm. My journey, marked by an insatiable curiosity and unyielding dedication, has led me to uncover the secrets of microbes that hold profound implications for human health. The foundations of my journey were laid at the prestigious King Mongkut’s Institute of Technology Ladkrabang, where I earned a B.Sc. in Industrial Microbiology. During this phase, my fascination with the potential of microorganisms to create impactful substances was ignited. My research at this stage focused on the screening of Streptomyces spp. strains with the capability to produce antimicrobial compounds. With a hunger for deeper insights, I pursued a M.Sc. in Medical Microbiology at Chulalongkorn University, Thailand. Here, my focus shifted to the realm of medical virology. The research took me into the intricate mechanisms of herpes simplex virus (HSV) infection in activated T lymphocytes. Undeterred by the challenges of a rigorous academic path, I earned a Ph.D. in Medical Microbiology (International Program) from the same esteemed institution. My research evolved into a comprehensive exploration of the human papillomavirus (HPV) and its intricate association with cervical cancer. There were two parts of my doctoral work: the research unraveled the multifaceted role of the microbiome (Bacteriome, Mycobiome, and Virome) in HPV-related cervical cancer while dissecting the functions of the pivotal HPV16 E1 protein in cervical carcinogenesis. These groundbreaking insights contributed significantly to our understanding of the factors underpinning this complex and devastating disease. My journey transcends academia, extending into a dynamic and impactful professional realm. Currently a lecturer in the Department of Microbiology at the Faculty of Public Health, Mahidol University, my expertise spans an array of many research areas, including anti-viral drug screening, cell-based evaluation of novel anticancer drug screening, molecular mechanisms of viral infection, and cancer development, as well as the exploration of bacteriome, mycobiome, and virome in diverse settings.
Positions
2023 Research Assistant (Department of Microbiology, Faculty of Medicine, Chulalongkorn University)
2015 Trainee (Department of Medical Sciences, Ministry of Public Health, Thailand)
EDUCATIONS
Bachelor of Science in Industrial Microbiology
Faculty of science, King Mongkut’s Institute of Technology Ladkrabang, Thailand
Learn More30/7/2016Master of Science in Medical Microbiology
Interdisciplinary Program, Graduate school, Chulalongkorn University, Thailand
Learn More14/1/2019Doctor of Philosophy (Medical Microbiology)
International Program, Graduate school, Chulalongkorn University, Thailand
Learn More23/12/2022Research Interests
Anti-viral drug screening
Anti-viral drug screening (Herpes simplex virus, Influenza virus, Human coronavirus, etc.) from natural products and microbial metabolites
Cell-based screening of novel anticancer drugs
Cervical cancer, melanoma, etc.
Molecular biology of viral infection and cancer development
Metagenomics
Observation of the bacteriome, mycobiome, and virome in clinical specimens, environment, food, etc.
PUBLICATIONS
- Sasivimolrattana T, Gunawan A, Wattanathavorn W, Pholpong C, Chaiwongkot A, Bhattarakosol P, et al. Upregulation of HPV16E1 and E7 expression and FOXO3a mRNA downregulation in high-grade cervical neoplasia. PeerJ. 2024;12:e18601.
- Wattanathavorn W, Seki M, Suzuki Y, Buranapraditkun S, Kitkumthorn N, Sasivimolrattana T, et al. Downregulation of LAMB3 Altered the Carcinogenic Properties of Human Papillomavirus 16-Positive Cervical Cancer Cells. Int J Mol Sci. 2024;25(5). Impact Factor (2023) = 5.6; Q1 (2023)
- Sasivimolrattana T, Bhattarakosol P. Impact of actin polymerization and filopodia formation on herpes simplex virus entry in epithelial, neuronal, and T lymphocyte cells. Front Cell Infect Microbiol. 2023;13:1301859. Impact Factor (2023) = 5.7; Q1 (2023)
- Siridechakorn I, Bhattarakosol P, Sasivimolrattana T, Anoma S, Wongwad E, Nuengchamnong N, et al. Inhibitory efficiency of Andrographis paniculata extract on viral multiplication and nitric oxide production. Scientific Reports. 2023;13(1):19738. Impact Factor (2023) = 4.997; Q1(Top10%) (2023)
- Sasivimolrattana T, Chaiwongkot A, Bhattarakosol P. HPV16E1 downregulation altered the cell characteristics involved in cervical cancer development. Scientific reports. 2023;13(1):18217. Impact Factor (2023) = 4.997; Q1(Top10%) (2023)
- Sasivimolrattana T, Chantratita W, Sensorn I, Chaiwongkot A, Oranratanaphan S, Bhattarakosol P, et al. Cervical Microbiome in Women Infected with HPV16 and High-Risk HPVs. International journal of environmental research and public health. 2022;19(22):14716. Impact Factor (2021) = 4.614; Q1 (2021)
- Sasivimolrattana T, Chantratita W, Sensorn I, Chaiwongkot A, Oranratanaphan S, Bhattarakosol P. Human Virome in Cervix Controlled by the Domination of Human Papillomavirus. Viruses. 2022;14(9):2066. Impact Factor (2021) = 5.518; Q1 (2021)
- Baedyananda F, Sasivimolrattana T, Chaiwongkot A, Varadarajan S, Bhattarakosol P. Role of HPV16 E1 in cervical carcinogenesis. Front Cell Infect Microbiol. 2022;12:955847. Impact Factor (2021) = 4.300; Q1 (2021)
- Sasivimolrattana T, Hansasuta P, Maneesri Le Grand S, Bhattarakosol P. Actin Polymerization Is Required for Filopodia Formation Supporting HSV-1 Entry into Activated T Cells. Current Microbiology. 2021;79(1):23. Impact Factor (2020) = 2.180; Q2 (2020)
POSTER PRESENTATIONS
1. Thanayod Sasivimolrattana, Arkom Chaiwongkot, and Parvapan Bhattarakosol. Role of HPV16E1 in tumor characteristics of cervical cancer cells. The 31st Annual Scientific Meeting, The Virology Association (Thailand). December 1-2, 2022
2. Thanayod Sasivimolrattana, Arkom Chaiwongkot, and Parvapan Bhattarakosol. Role of HPV16E1 in tumor characteristics of cervical cancer cells. The 1st MD-Micro Research Day, Medical Microbiology, Interdisciplinary Program, Graduate school, Chulalongkorn University, Thailand. November 24, 2022
3. Thanayod Sasivimolrattana, Wasun Chantratita, Insee Sensorn, Arkom Chaiwongkot, Shina Oranratanaphan, Parvapan Bhattarakosol. Human Papillomavirus Domination Controls Cervical Virome. The Graduate Research Competition 2022, Faculty of Medicine, Chulalongkorn University, Thailand. August 25, 2022 (Oral presentation)
4. Thanayod Sasivimolrattana, Wasun Chantratita, Insee Sensorn, Arkom Chaiwongkot, Shina Oranratanaphan, Parvapan Bhattarakosol. Relationship between cervical virome and cytological characteristics of high-risk HPVs infected patients. MDCU Congress 2022, Faculty of Medicine, Chulalongkorn University, Thailand. August 17-19, 2022
5. Thanayod Sasivimolrattana, Wasun Chantratita, Insee Sensorn, Arkom Chaiwongkot, Shina Oranratanaphan, Parvapan Bhattarakosol. Relationship between cervical virome and cytological characteristics of high-risk HPVs infected patients. Keystone Symposia eSymposia meeting Global Priorities in Vaginal Health: Microbes, Mucosal Immunity, and Interventions. November 10-12, 2021
6. Thanayod Sasivimolrattana and Parvapan Bhattarakosol. Filopodia formation plays a key role in HSV-1 entry in activated T lymphocytes. Proceeding of the 28th Annual Scientific Meeting, The Virology Association (Thailand). November 15-16, 2018
2. Thanayod Sasivimolrattana, Arkom Chaiwongkot, and Parvapan Bhattarakosol. Role of HPV16E1 in tumor characteristics of cervical cancer cells. The 1st MD-Micro Research Day, Medical Microbiology, Interdisciplinary Program, Graduate school, Chulalongkorn University, Thailand. November 24, 2022
3. Thanayod Sasivimolrattana, Wasun Chantratita, Insee Sensorn, Arkom Chaiwongkot, Shina Oranratanaphan, Parvapan Bhattarakosol. Human Papillomavirus Domination Controls Cervical Virome. The Graduate Research Competition 2022, Faculty of Medicine, Chulalongkorn University, Thailand. August 25, 2022 (Oral presentation)
4. Thanayod Sasivimolrattana, Wasun Chantratita, Insee Sensorn, Arkom Chaiwongkot, Shina Oranratanaphan, Parvapan Bhattarakosol. Relationship between cervical virome and cytological characteristics of high-risk HPVs infected patients. MDCU Congress 2022, Faculty of Medicine, Chulalongkorn University, Thailand. August 17-19, 2022
5. Thanayod Sasivimolrattana, Wasun Chantratita, Insee Sensorn, Arkom Chaiwongkot, Shina Oranratanaphan, Parvapan Bhattarakosol. Relationship between cervical virome and cytological characteristics of high-risk HPVs infected patients. Keystone Symposia eSymposia meeting Global Priorities in Vaginal Health: Microbes, Mucosal Immunity, and Interventions. November 10-12, 2021
6. Thanayod Sasivimolrattana and Parvapan Bhattarakosol. Filopodia formation plays a key role in HSV-1 entry in activated T lymphocytes. Proceeding of the 28th Annual Scientific Meeting, The Virology Association (Thailand). November 15-16, 2018
CERTIFICATES
SELECTED CERTIFICATES
- Fundamental Biosafety for BSL-2, Faculty of Science, Mahidol University, Thailand (2024)
- Professional Researcher Empowerment Program (PREP Batch 7), Mahidol University, Thailand (2023)
- Certificate of Participation (Chair), International Conferences for Public Health, Environment, and Education for Sustainable Development Goals and Lifelong Learning, Faculty of Public Health, Mahidol University, Thailand (2023)
- Metagenomic Next-Generation Sequencing (mNGS), Department of Medical Sciences, Ministry of Public Health, Thailand (2023)
- The use of animal models in scientific experiments, National Research Council of Thailand (NRCT), Thailand (2023)
- Ethics in Human Research, Ethical Review, Committee for Human Research Faculty of Public health, Mahidol University, Thailand (2023)
- Certificate of attendance in the workshop “The ripple effect of COVID-19 pandemic on respiratory virus research and healthcare: Genetic analysis of influenza viruses and SARS-CoV-2”, Center of disease control and disease prevention (CDC), and the virology association, Thailand (2023)
- 16s rRNA gene sequencing from samples to analysis, the Microbiome Workshop, the Omics Sciences and Bioinformatics Center, Faculty of Science, Chulalongkorn University (2023)
- Biosafety Guidelines, Center for Safety, Health and Environment of Chulalongkorn University (SHECU), Thailand (2021)
- Bioinformatic Methods I and II, an online non-credit course through Coursera by University of Toronto, Canada (2020)
- Academic English: Writing (Grammar and Punctuation), an online non-credit course through Coursera by University of California, Irvine (UCI), USA (2020)
- English for Research Publication Purposes (Academic Literacy), an online non-credit course through Coursera by Moscow Institute of Physics and Technology (MIPT), Russia (2020)
- Chemical Safety Training for Research Students and Researchers, Center for Safety, Health and Environment of Chulalongkorn University (SHECU), Thailand (2020)
- Media and Biochemical Identification of Intestinal Pathogens, Bureau of Personnel Development, Department of Science Service, Ministry of Science and Technology, Thailand (2016)
LABORATORY SKILLS
Cell Culture
Subculture of adherent and suspension cells, Cell isolation, Cell cytotoxicity assay (MTS), Viable cell count, T cell activation, Transfection, RNA interference (siRNA), Wound healing assay, Cell migration/invasion assay, Clonogenic assay, Soft agar colony formation assay, Flow cytometry analysis
Virological methods
Viral culture/propagation, Viral isolation, Viral plaque titration assay
PCR
Reverse transcription, Conventional PCR, Mutiplex PCR, Real-time PCR
Protein assays
Protein extraction, Protein measurement (Bradford assay), SDS-PAGE, Western blot (WB)
Microscopic Methods
Immunofluorescence assay, Sample preparation for Transmission electron microscopy (TEM)
Next-Generation Sequencing (NGS)
Libraries preparation (Swift amplicons and SeqCap EZ HyperCap), Fragment analysis, Illumina sequencing (Illumina MiSeq)
Bioinformatics
Ubuntu/Docker (VIP; Viral Identification Pipeline), Basic command line, UGENE, QIAGEN CLC Genomics Workbench software (Microbial Genomics Module), BaseSpace Sequence Hub (16S rRNA/ITS Metagenomics Module and DRAGEN Metagenomics Pipeline) of Illumina
Microbiological assays
Bacterial and Fungal culture/isolation and biochemical test, Agar disk diffusion assay, Slide culture technique (Actinomycetes)
Other Methods in Molecular biology
DNA/RNA measurement (Qubit and Nanodrop), DNA/RNA extraction, second strand cDNA synthesis
Software
Microsoft office, GraphPad Prism, PAST statistical, Adobe (Illustrator, Lightroom, Audition), EndNote, ImageJ, Biorender.com
SPEAKER
โครงการแลกเปลี่ยนเรียนรู้ Presentation Tips & การตกแต่งสื่อการเรียนการสอนด้วย MS Office 365 ภายใต้โครงการ PH Pride Heroes
Organized by Faculty of Public Health, Mahidol University
งานเสวนาวิชาการ: MPOX รู้ทัน พร้อมรับมือ (2024)
Organized by Faculty of Public Health, Mahidol University // Co-organized by Faculty of Science, Mahidol University; Faculty of Tropical Medicine, Mahidol University; Department of Disease Control Ministry of Public Health
International conference: Medlab Asia (2024)
Topic: Human virome in health and diseases // Organized by Informa Markets
Special seminar: Seminar in Medical Microbiology at KKU (2024)
Topic: HPV16 E1 downregulated altered the cell characteristics involved in cervical cancer development // Course: Seminar in Medical Microbiology // Organized by Department of Microbiology, Faculty of Medicine, Khon Kaen University
Special guidance: The road to Ph.D. and M.Sc. (2020)
Organized by Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang (KMITL)
TEACHING EXPERIENCES
- 2023 - present: Lecturer at Department of Microbiology, Faculty of Public Health, Mahidol University, Thailand
- 2023: Invited lecturer for Biology of Viruses course at Faculty of Medicine, Chulalongkorn University
- 2017-2018 and 2019-2020: Teacher assistant for medical students in Microbiology laboratory, Faculty of medicine, Chulalongkorn university
- 2017-2018 and 2019-2020: Teacher assistant of Techniques and Applications in Molecular Biology for Ph.D. and M.Sc. students in Medical Microbiology, Interdisciplinary Program, Chulalongkorn university
AWARDS
- “FIRST PRIZE” for the oral presentation “Role of HPV16E1 in tumor characteristics of cervical cancer cells” on the 1st MD-Micro Research Day, Medical Microbiology, Interdisciplinary Program, Graduate school, Chulalongkorn University, Thailand. November 24th, 2022
- “FIRST PRIZE” for the oral presentation “Human Papillomavirus Domination Controls Cervical Virome” in the category of “MSc/PhD students” at the Graduate Research Competition 2022, Faculty of Medicine, Chulalongkorn University, Thailand. August 25th, 2022
- “SILVER MEDAL” for the invention “Anti-microbial hand cream with Andrographis paniculata extract” at the 2022 Japan Design, Idea & Invention Expo, Kyoto, Japan. July 1st-3rd, 2022
- “GOOD AWRD” for the oral presentation “HSV-1 Promotes Filopodia Formation via Upregulation of Cdc42 to Support Its Entry into Activated T Cells” in the category of “MSc/PhD students” at the Graduate Research Competition 2019, Faculty of Medicine, Chulalongkorn University, Thailand. August 15th, 2019
coOperations
Division of Virology, Department of Microbiology, Faculty of Medicine, Chulalongkorn University
Read More
Faculty of Medicine, Ramathibodi Hospital, Mahidol University
Read More
Grants
The 100th Anniversary Chulalongkorn University Fund for Doctoral Scholarship
฿417000
The 90th Anniversary Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund)
฿225000
Strategic Research Fund 2024, Mahidol University
฿600000
ARCHIVES
- Former tenor at KMITL Chorus
- Part-time singer
